Please contact us (miriam.vitt (at) bioss.uni-freiburg.de) if you are interested in use of the equipment.
Cytek Aurora spectral flow cytometer
Technical specifications:
5 lasers: UV (355 nm), violet (405 nm), green (488 nm), yellow-green (561 nm) and red (640 nm).
Detectors: 64 detectors for efficient spectrum capture in the 365 – 829 nm range.
The set up allows simultaneous detection of up to 40 fluorophores.
Additional: high-throughput auto-sampler.
The instrument is fully set-up for real-time analysis of cell signaling (for example, calcium flux).
More information can be found here.
Visitron High Power TIRF/FRAP/EPI fluorescence Microscope
Capabilities:
(i) Total Internal Reflection Microscopy, where illumination is limited to the contact site between cells and glass surfaces.
(ii) Single molecule detection for super resolution imaging and particle tracking to analyze protein distributions and dynamics. Images are acquired with two CMOS cameras with high dynamic range and large fields of view.
(iii) Localized laser control for photo-manipulation to measure protein dynamics and dispersion. Up to six medium powered lasers covering a broad range of illumination wavelengths.
(iv) Widefield illumination for fast acquisition and calcium analyses.
(v) Environmental control for live cell imaging.
Key components:
Microscope: Inverted high-end microscope (Ti2-E Dual Layer)
Objectives: 100x TIRF
60x TIRF
40x Fluor (UV transmission)
Illumination: Azimuthal, spinning-spot or ring TIRF (Visitron Orbital 500)
Photomanipulation, Instant Switching of Illumination Modes
Laser (200-500mW): 405nm, 488nm, 561nm and 640nm
Camera: CMOS with large field of view (dual camera system)
Photometrix Kinetix 22
Wide-Field: CoolLED pE-800 Fura
Stage: High precision and high reproducibility XYZ
Incubation system: Life Imaging Box system
(high stability of temperature and humidity)
Visitron Low Power TIRF/FRAP/EPI fluorescence Microscope
Capabilities:
(i) Total Internal Reflection Microscopy, where illumination is limited to the contact site between cells and glass surfaces.
(ii) Single molecule detection for super resolution imaging and particle tracking to analyze protein distributions and dynamics. Images are acquired with two CMOS cameras with high dynamic range and large fields of view.
(iii) Localized laser control for photo-manipulation to measure protein dynamics and dispersion. Up to six medium powered lasers covering a broad range of illumination wavelengths.
(iv) Widefield illumination for fast acquisition and calcium analyses.
(v) Environmental control for live cell imaging.
Key components:
Microscope: Inverted high-end microscope (Ti2-E Dual Layer)
Objectives: 100x TIRF
60x TIRF
40x Fluor (UV transmission)
Illumination: Azimuthal, spinning-spot or ring TIRF (Visitron Orbital 200)
Photomanipulation, Instant Switching of Illumination Modes
Laser (100-150mW): 405nm, 445nm, 488nm, 515nm, 561nm and 640nm
Camera: CMOS with large field of view (dual camera system)
Photometrix 95B
Wide-Field: CoolLED pE-800 Fura
Stage: High precision and high reproducibility XY
Incubation system: Life Imaging Box system
(high stability of temperature and humidity)
ÄKTA pure chromatography system
A versatile and modular system for protein purification. More information can be found here.
Infinite M Plex microplate reader
Detection modes: absorbance, fluorescence, luminescence
Wavelength range: Absorbance: 230 nm – 1000 nm, FI Excitation: 230 nm – 850 nm, FI Emission: 280 nm – 850 nm, Glow type luminescence: 380 nm – 600 nm
More information can be found here.
